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pbad mtfp1  (Addgene inc)


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    Structured Review

    Addgene inc pbad mtfp1
    Pbad Mtfp1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbad+mtfp1/mTFP1-pBAD+(Plasmid+%2354553)/bio_rxiv__2025__10__27__684934-252-38-39
    Average 92 stars, based on 8 article reviews
    pbad mtfp1 - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Synthetic multicolor antigen-stabilizable nanobody platform for intersectional labelling and functional imaging
    Article Snippet: VIS-Fbs to GFP with insertions of mTagBFP2, mTFP1, mWasabi, mNeonGreen, mOrange, LSSmOrange, CyOFP1, mScarlet-I, mScarlet, LSSmScarlet, mNeptune2, mCardinal, PAmCherry or mEos4a were designed by swapping miRFP670nano3 gene with FPs genes in the NIR-Fb plasmid (Addgene no.184675) by NEBuilder HiFi DNA Assembly Kit. .. All oligonucleotide primers for PCR amplification were purchased from Thermo Fisher Scientific ( Supplementary Table 1 ). mTagBFP2, mTFP1, mWasabi, mNeonGreen, mOrange, LSSmOrange, CyOFP1, mScarlet-I, mScarlet, LSSmScarlet, mNeptune2, mCardinal, PAmCherry, and mEos4a were amplified from pBAD-mTagBFP2 (Addgene no.34632), pBAD-mTFP1 (Addgene no.54553), pmWasabi-FAK-5 (Addgene no.56502), pmOrange-N1 (Addgene no.54499), pBAD-LSSmOrange (Addgene no.37129), pKK-CyOFP1-TEV (Addgene no.105782), pmScarlet-I-C1 (Addgene no.85044), pmScarlet-C1 (Addgene no.85042), pcDNA3-mNeptune2 (Addgene no.51309), pcDNA3-mCardinal (Addgene no.51311), PAmCherry1-C1 (Addgene no.54495) and mEoa4a-N1 (Addgene no.54811) plasmids, respectively. .. To generate the NES-mTagBFP2-Fb plasmid, the mTagBFP2-Fb GFP gene was swapped with DEVD-mCardinal-3xNLS construct in the pcDNA-NES-DEVD-mCardinal-3xNLS plasmid (Addgene no.164052) at the BamHI/XhoI sites.

    Amplification:

    Article Title: Synthetic multicolor antigen-stabilizable nanobody platform for intersectional labelling and functional imaging
    Article Snippet: VIS-Fbs to GFP with insertions of mTagBFP2, mTFP1, mWasabi, mNeonGreen, mOrange, LSSmOrange, CyOFP1, mScarlet-I, mScarlet, LSSmScarlet, mNeptune2, mCardinal, PAmCherry or mEos4a were designed by swapping miRFP670nano3 gene with FPs genes in the NIR-Fb plasmid (Addgene no.184675) by NEBuilder HiFi DNA Assembly Kit. .. All oligonucleotide primers for PCR amplification were purchased from Thermo Fisher Scientific ( Supplementary Table 1 ). mTagBFP2, mTFP1, mWasabi, mNeonGreen, mOrange, LSSmOrange, CyOFP1, mScarlet-I, mScarlet, LSSmScarlet, mNeptune2, mCardinal, PAmCherry, and mEos4a were amplified from pBAD-mTagBFP2 (Addgene no.34632), pBAD-mTFP1 (Addgene no.54553), pmWasabi-FAK-5 (Addgene no.56502), pmOrange-N1 (Addgene no.54499), pBAD-LSSmOrange (Addgene no.37129), pKK-CyOFP1-TEV (Addgene no.105782), pmScarlet-I-C1 (Addgene no.85044), pmScarlet-C1 (Addgene no.85042), pcDNA3-mNeptune2 (Addgene no.51309), pcDNA3-mCardinal (Addgene no.51311), PAmCherry1-C1 (Addgene no.54495) and mEoa4a-N1 (Addgene no.54811) plasmids, respectively. .. To generate the NES-mTagBFP2-Fb plasmid, the mTagBFP2-Fb GFP gene was swapped with DEVD-mCardinal-3xNLS construct in the pcDNA-NES-DEVD-mCardinal-3xNLS plasmid (Addgene no.164052) at the BamHI/XhoI sites.



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